Topics related by tag: Oncology
Microenvironmental signals control hybrid EMT phenotypes
A Scaffold Simulator to study of cancer cell survival and 3D spatial distribution (SALSA)
Computational models of Epithelial to Mesenchymal Transition
Computational representation of complex biological processes is becoming an increasingly established approach to complement the experimental analysis and study methods to halt/redirect pathological processes. In this review paper we focus on Epithelial to Mesenchymal transition (EMT), a phenotypic transformation with a pivotal role in cancer progression and ...
Computational model of cell phenotype decision making
Epithelial to Mesenchymal transition (EMT) is an exceedingly complex biological process that plays a key role in cancer progression and metastases formation. To unravel this complexity and isolate specific genetic markers important for this transition, we have developed a computational model recapitulating both single cell and population behaviours. The former was ...
Semi-Automatic Analysis of Invasion and Migration Transwell Assays (I-AbACUS)
Transwell assays are another common experimental approach for the quantification of migration and invasion (see also Automatic quantification of (cancer) cell invasiveness). This method relies on a semi-permeable membrane and a layer of ...
Automatic quantification of (cancer) cell invasiveness
Quantification of (cancer) cell invasiveness can be obtained through the "scratch wound healing assay", a simple experimental framework evaluating the rate at which the tested cells are able to fill a gap expressly produced in a confluent culture. Despite its being widely used, the approaches used to analyse this in-vitro assay lack standardization and do not allow ...