Wednesday, 21 Apr 2021

Topics related by tag: Recombinant DNA technology

Mammalian synthetic biology

Mammalian cells are important tools for bioprocessing applications and for investigating the mechanisms of cancer development. Although a close relation has been observed in these systems between cellular growth and the production of intracellular resources needed for transcription and translation, quantitative empirical relations are still lacking. Investigating ...

Phenotypic variability in synthetic biology applications

The stochasticity due to the infrequent collisions among low copy-number molecules within the crowded cellular compartment is a feature of living systems. Single cell variability in gene expression within an isogenic population (i.e. biological noise) is usually described as the sum of two independent components: intrinsic and extrinsic stochasticity. Intrinsic ...

Mathematical models predict the behavior of modular synthetic gene circuits

Most of synthetic circuits developed have been designed by an ad hoc approach, using a small number of components (i.e. LacI, TetR) and a trial and error strategy. We are at the point where an increasing number of modular, inter-changeable and well-characterized components is needed to expand the construction of synthetic devices and to allow a rational approach to ...

Synthetic post-transcriptional control of gene expression

Post-transcriptional regulation allows a faster control of gene expression, which might result relevant in the design of synthetic gene circuits. However, synthetic biologists are warned to give the proper consideration to functional modularity of biological parts and the need for a case-to-case characterization of their function, raising concern about the use of a ...

Quantification of fluorescent protein markers in single bacterial cell

The evaluation of phenotypic variability within an isogenic population of bacterial cells, requires the quantification of gene expression at single cell level. Flow cytometers and microscopy set-ups equipped with microfluidic devices and incubation chambers are instruments capable of evaluating the level of specific proteins in individual cells. However they are ...