Saturday, 15 May 2021
Marilisa Cortesi

Marilisa Cortesi

Biomedical engineer graduated magna cum laude at the University of Bologna, Italy, in 2013. In 2017 she was awarded a PhD in Bioengineering from the same institution, with a thesis on computational modelling of complex biological processes and their application to the identification of potential therapeutic targets. More

A Scaffold Simulator to study of cancer cell survival and 3D spatial distribution (SALSA)

3D cell cultures are becoming increasingly common in-vitro approaches to study cell behaviour in more accurate and realistic settings. The experimental study of these systems, however, is complicated by the lack of non-destructive methods for the quantification of relevant properties like cell density and spatial distribution. Computational simulations can be used to address this limitation and ...

Computational models of Epithelial to Mesenchymal Transition

Computational representation of complex biological processes is becoming an increasingly established approach to complement the experimental analysis and study methods to halt/redirect pathological processes. In this review paper we focus on Epithelial to Mesenchymal transition (EMT), a phenotypic transformation with a pivotal role in cancer progression and metastases formation, and present a ...

Computational model of cell phenotype decision making

Epithelial to Mesenchymal transition (EMT) is an exceedingly complex biological process that plays a key role in cancer progression and metastases formation. To unravel this complexity and isolate specific genetic markers important for this transition, we have developed a computational model recapitulating both single cell and population behaviours. The former was represented with a boolean ...

Semi-Automatic Analysis of Invasion and Migration Transwell Assays (I-AbACUS)

Transwell assays are another common experimental approach for the quantification of migration and invasion (see also Automatic quantification of (cancer) cell invasiveness). This method relies on a semi-permeable membrane and a layer of Matrigel coating to simulate ...

Automatic quantification of (cancer) cell invasiveness

Quantification of (cancer) cell invasiveness can be obtained through the "scratch wound healing assay", a simple experimental framework evaluating the rate at which the tested cells are able to fill a gap expressly produced in a confluent culture. Despite its being widely used, the approaches used to analyse this in-vitro assay lack standardization and do not allow for an accurate and objective ...

Quantification of fluorescent protein markers in single bacterial cell

The evaluation of phenotypic variability within an isogenic population of bacterial cells, requires the quantification of gene expression at single cell level. Flow cytometers and microscopy set-ups equipped with microfluidic devices and incubation chambers are instruments capable of evaluating the level of specific proteins in individual cells. However they are expensive and generally ...